2-step plus Poly-HRP Anti Rabbit/Mouse IgG Detection System
Price: $ 400
Price: $ 180
Price: $ 100
Size:
18mL
6mL
3mL
Quantity:
- Applications: IHC
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For research use only. Order now, ship in 3 days
Product Details
Product Introduction | 2-step plus is a two-step immunohistochemical broad spectrum detection reagent. It polymerizes monovalent Fab fragments of secondary antibody and enzyme, which replaces the secondary antibody and tertiary antibody in traditional method, can directly amplify the binding signal of antibody-antigen. This method not only retains the specific binding ability of antibody with antigen, but also can effectively avoid space steric hindrance caused by excessive polymer molecules. Compared with the traditional SP three-step method, this kit has the characteristics of simple, rapid, high-sensitivity. This system abandons the using of biotin, so it can avoid background staining by endogenous biotin. It can be used in IHC, in which the primary antibody is monoclonal/polyclonal antibody derived from rabbit/mouse. The unique polymer auxiliary agent for macromolecular detection system can detect the binding primary antibody better. |
Application | IHC |
Storage | Store at 2~8°C, shading light. Avoid of freezing. Valid for 12 months. The reagents are valid within 6 months after opening. |
Shipping | Biological ice pack at 4 ℃ |
Cautions |
1. This product couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence. 2. For your safety and health, please wear the lab coat and disposable gloves before the experiments. |
Citations
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RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation
IF:8.44
Journal:Journal of Translational Medicine
DOI:10.1186/s12967-022-03526-0
PMID:35879796
-
Nocardia rubra cell-wall skeleton influences the development of cervical carcinoma by promoting the antitumor effect of macrophages and dendritic cells
IF:4.452
Journal:Cancer Medicine
PMID:34994088
-
Enhancing osteogenic potential of hDPSCs by resveratrol through reducing oxidative stress via the Sirt1/Nrf2 pathway
IF:3.503
Journal:PHARMACEUTICAL BIOLOGY
DOI:10.1080/13880209.2022.2037664
PMID:35188840
-
Microglial activation and neurobiological alterations in experimental autoimmune prostatitis-induced depressive-like behavior in mice
IF:2.228
Journal:Neuropsychiatric Disease and Treatment
PMID:31496706
-
GABRD promotes progression and predicts poor prognosis in colorectal cancer
IF:1.204
Journal:Open Medicine
Experiment Operation Video
Protocols
• Western Blot Guide
• Immunohistochemistry Guide for Slide-mounted Paraffin Sections
• Immunofluorescence Guide
• Immunohistochemistry Troubleshooting Tips
• Western Blot Troubleshooting Tips
• Immunofluorescence Troubleshooting Tips
• Comparison of Protein Separation Linear Range
• ImmunoPrecipitation Protocol