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8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit

  • Cat.No.:E-EL-0041

  • Reactivity: Universal

To Purchase E-EL-0041

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes 8-epi-PGF2α in samples. No significant cross-reactivity or interference between 8-epi-PGF2α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 8-epi-PGF2α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 8-epi-PGF2α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.20 101.80 446.40 54.50 95.70 463.70
Standard deviation 2.90 4.50 15.60 3.50 5.00 25.00
CV (%) 5.66 4.42 3.49 6.42 5.22 5.39


The recovery of 8-epi-PGF2α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 92
EDTA plasma(n=8) 92-108 100
Cell culture media(n=8) 93-107 100


Samples were spiked with high concentrations of 8-epi-PGF2α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms15-F2t-IsoP, 8-iso-PGF2a, 8-isoprostane, 8-iso-PG, 8-iso Prostaglandin
Research AreaSignal transduction, Cardiovascular

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nutrients (2020) IF: 4.546
    Total Dietary Antioxidant Intake Including Polyphenol Content: Is it Capable to Fight against Increased Oxidants within the Body of Ultra-Endurance Athletes?

    DOI: 10.3390/nu12061877

    PMID: 32586010

    Sample: Plasma
  2. Medical Archives (2020) IF: 2.807
    Trimetazidine Improves the Outcome of EECP Therapy in Patients with Refractory Angina Pectoris

    DOI: 10.5455/medarh.2020.74.199-204

    PMID: 32801436

    Sample: Serum
  3. Journal of Diabetes Research (2015) IF: 2.717
    Edible Bird'S Nest Prevents High Fat Diet-Induced Insulin Resistance In Rats.

    DOI: 10.1155/2015/760535

    PMID: 26273674

    Sample: Serum
  4. Food & Nutrition Research (2016) IF: 2.039
    Perinatal Exposure To Germinated Brown Rice And Its Gamma Amino-Butyric Acid-Rich Extract Prevents High Fat Diet-Induced Insulin Resistance In First Generation Rat Offspring.

    DOI: 10.3402/fnr.v60.30209

    PMID: 26842399

    Sample: Serum
  5. Life Sciences (2017) IF: 2.936
    Roselle Is Cardioprotective In Diet-induced Obesity Rat Model With Myocardial Infarction.

    DOI: 10.1016/j.lfs.2017.10.030

    PMID: 29066253

    Sample: Tissue homogenate
  6. Al-Qadisiyah Journal Of Pure Science (2020)
    Roles of Superoxide dismutase(SOD) , Malondialdehyde (MDA), 8-iso-prostaglandinF2α ( 8-iso-PGF2α)as oxidative stress in development and progression of Brest cancer in Iraqi females patients

    DOI: 10.29350/jops.2020.25.1.1053

    Sample: Plasma
  7. Journal of dental research, dental clinics, dental prospects (2018)
    8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link

    DOI: 10.15171/joddd.2018.039

    PMID: 30774790

    Sample: Saliva
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