Current Position:Home>>Kits>>ELISA Kits>> 8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit

8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit

Cat.No.:E-EL-0041
Reactivity: Universal

Filter:

ELISA

To Purchase E-EL-0041

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes 8-epi-PGF2α in samples. No significant cross-reactivity or interference between 8-epi-PGF2α and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 8-epi-PGF2α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 8-epi-PGF2α were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.20	101.80	446.40	54.50	95.70	463.70
Standard deviation	2.90	4.50	15.60	3.50	5.00	25.00
CV (%)	5.66	4.42	3.49	6.42	5.22	5.39

Recovery

The recovery of 8-epi-PGF2α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-97	92
EDTA plasma(n=8)	92-108	100
Cell culture media(n=8)	93-107	100

Linearity

Samples were spiked with high concentrations of 8-epi-PGF2α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	15-F2t-IsoP, 8-iso-PGF2a, 8-isoprostane, 8-iso-PG, 8-iso Prostaglandin
Research Area	Signal transduction, Cardiovascular

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Nutrients (2020) IF: 4.546

Total Dietary Antioxidant Intake Including Polyphenol Content: Is it Capable to Fight against Increased Oxidants within the Body of Ultra-Endurance Athletes?

DOI: 10.3390/nu12061877
PMID: 32586010	Sample: Plasma

Medical Archives (2020) IF: 2.807

Trimetazidine Improves the Outcome of EECP Therapy in Patients with Refractory Angina Pectoris

DOI: 10.5455/medarh.2020.74.199-204
PMID: 32801436	Sample: Serum

Journal of Diabetes Research (2015) IF: 2.717

Edible Bird'S Nest Prevents High Fat Diet-Induced Insulin Resistance In Rats.

DOI: 10.1155/2015/760535
PMID: 26273674	Sample: Serum

Food & Nutrition Research (2016) IF: 2.039

Perinatal Exposure To Germinated Brown Rice And Its Gamma Amino-Butyric Acid-Rich Extract Prevents High Fat Diet-Induced Insulin Resistance In First Generation Rat Offspring.

DOI: 10.3402/fnr.v60.30209
PMID: 26842399	Sample: Serum

Life Sciences (2017) IF: 2.936

Roselle Is Cardioprotective In Diet-induced Obesity Rat Model With Myocardial Infarction.

DOI: 10.1016/j.lfs.2017.10.030
PMID: 29066253	Sample: Tissue homogenate

Al-Qadisiyah Journal Of Pure Science (2020)

Roles of Superoxide dismutase(SOD) , Malondialdehyde (MDA), 8-iso-prostaglandinF2α ( 8-iso-PGF2α)as oxidative stress in development and progression of Brest cancer in Iraqi females patients

DOI: 10.29350/jops.2020.25.1.1053
	Sample: Plasma

Journal of dental research, dental clinics, dental prospects (2018)

8-Isoprostane in chronic periodontitis and type II diabetes: Exploring the link

DOI: 10.15171/joddd.2018.039
PMID: 30774790	Sample: Saliva

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha