8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
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    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-0028

      Synonyms:7,8-Dihydro-8-oxo-2'-deoxyguanosine; 7,8-Dihydro-8-oxodeoxyguanosine; 8-Hydroxy-2'-deoxyguanosine; 8-Hydroxydeoxyguanosine; 8-Oxo-2'-deoxyguanosine; 8-Oxo-7,8-dihydro-2'-deoxyguanosine; 8-Oxo-7,8-dihydrodeoxyguanosine; 8-Oxo-dG; 8-OH-dG

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Universal

      Detection Range: 1.56~100 ng/mL

      Sensitivity: 0.94 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of  8-OHdG concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-OHdG. During the reaction,  8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  8-OHdG. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

      Assay type Competitive
      Format 96T
      Assay time 2.0h
      Reactivity Universal
      Detection Method Colormetric
      Detection Range 1.56—100 ng/mL
      Sensitivity 0.94 ng/mL
      Sample Volume 50μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes 8-OHdG in samples. No significant cross-reactivity or interference between 8-OHdG and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(ng/mL) O.D Average
      100 0.18
      0.224
      0.202
      50 0.33
      0.354
      0.342
      25 0.568
      0.542
      0.555
      12.5 0.895
      0.905
      0.9
      6.25 1.367
      1.345
      1.356
      3.13 1.772
      1.75
      1.761
      1.56 2.1
      2.104
      2.102
      0 2.597
      2.599
      2.598

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 8-OHdG were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 8-OHdG were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (ng/mL) 4.79 10.11 46.98 4.48 9.61 48.89
      Standard deviation 0.27 0.58 1.69 0.27 0.55 1.91
      C V (%) 5.66 5.69 3.60 5.95 5.70 3.90

      Recovery

      The recovery of  8-OHdG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 86-100 91
      EDTA plasma (n=5) 91-104 98
      Cell culture media (n=5) 93-106 98

      Linearity

      Samples were spiked with high concentrations of 8-OHdG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 92-104 87-101 90-103
      Average (%) 98 92 95
      1:4 Range (%) 89-106 82-94 83-95
      Average (%) 97 88 90
      1:8 Range (%) 93-106 85-100 88-99
      Average (%) 100 92 94
      1:16 Range (%) 87-102 86-99 88-101
      Average (%) 94 92 96

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

      elisa assay procedure 2

      2. Aspirate and wash the plate for 3 times

      elisa assay procedure 3

      3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 4

      4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 5

      5. Add 50μL Stop Solution

      elisa assay procedure 6

      6. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Chronic Obstructive Pulmonary Disease-Derived Circulating Cells Release IL-18 And IL-33 Under Ultrafine Particulate Matter Exposure In A Caspase-1/8-Independent Manner.
        IF: 6.429
        Journal: Frontiers in Immunology(2017)
        DOI: 10.3389/fimmu.2017.01415
        PMID: 29123531
        Products: E-EL-0028 
        Sample type: Cell culture supernatant
        Reactivity: Universal
      2. Cerium Oxide Nanoparticles Promote Neurogenesis And Abrogate Hypoxia-Induced Memory Impairment Through AMPK-PKC-CBP Signaling Cascade.
        IF: 4.3
        Journal: International Journal of Nanomedicine(2016)
        DOI: 10.2147/IJN.S102096
        PMID: 27069362
        Products: E-EL-0028 
        Sample type: Tissue homogenate
        Reactivity: Rat
      3. A Comparative Study On Antioxidant System In Fish Hepatopancreas And Intestine Affected By Choline Deficiency: Different Change Patterns Of Varied Antioxidant Enzyme Genes And Nrf2 Signaling Factors.
        IF: 2.806
        Journal: Plos One(2017)
        DOI: 10.1371/journal.pone.0169888
        PMID: 28099509
        Products: E-EL-0028 
        Sample type: Tissue homogenate
        Reactivity: Universal
      4. Elevated Serum Levels Of 8-Hydroxy-2-Deoxyguanosine In Mild-Moderate Acne Vulgaris.
        Journal: Medicine Science(2018)
        DOI: 10.5455/medscience.2017.06.8705
        Products: E-EL-0028 
        Reactivity: Universal
      5. Toxicological Studies On Heavy Metal Bioaccumulation And Oxidative DNA Damage In Residents Of An Oil Producing Area.
        IF: /
        Journal: International Journal of Scientific & Technology research(2018)
        Products: E-EL-0028 
        Sample type: Serum
        Reactivity: Human
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