Current Position:Home>>Kits>>Metabolism Assays>> Ferrous Iron Colorimetric Assay Kit

Ferrous Iron Colorimetric Assay Kit

Cat.No.:E-BC-K304-S
Detection instrument: Spectrophotometer(520 nm)

Detection method: Colorimetric method

To Purchase E-BC-K304-S

Size:

100 Assays
50Assays

Price: $220

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.

Performance characteristics

Sample type	Serum, plasma, animal tissue
Sensitivity	0.08 mg/L
Detection range	0.08-20 mg/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	45 min
Precision	Average inter-assay CV: 3.200%Average intra-assay CV: 3%
Other instruments required	Test tubes, Vortex Mixer, Centrifuge, Water bath, Test tubes, Vortex Mixer, Centrifuge, Water bath
Storage	2-8℃
Valid period	12 months

Images

The administration of Fe nanoparticle significantly increased the ferrous level in cells with dose dependent.

T Tong et al developed a novel ferrous sulfide nanoparticles for inhibiting porcine reproductive and respiratory syndrome virus. Ferrous ion levels in cell were determined using ferrous ion colorimetric assay kit (E-BC-K304-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.08-50 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
Human serum	1
10% Rat liver tissue homogenate	1
10% Epipremnum aureum homogenate	1

Note: The diluent is double distilled water).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
Inhibitory effect and mechanism of gelatin stabilized ferrous sulfide nanoparticles on porcine reproductive and respiratory syndrome virus

DOI: 10.1186/s12951-022-01281-4

PMID: 35123507
Sample: MARC-145 cell
Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization

DOI: 10.1155/2022/9735555

PMID: 35993021
Sample: Kidney tissue,HK-2 cell
EXPERIMENTAL NEUROLOGY (2022) IF: 5.62
S100A8 regulates autophagy-dependent ferroptosis in microglia after experimental subarachnoid hemorrhage

DOI: 10.1016/j.expneurol.2022.114171

PMID: 35870523
Sample: Brain tissue,BV2 microglia
Frontiers in Oncology (2022) IF: 5.738
Circular RNA circLMO1 Suppresses Cervical Cancer Growth and Metastasis by Triggering miR-4291/ACSL4-Mediated Ferroptosis

DOI: 10.3389/fonc.2022.858598

Sample: CaSki cell
Frontiers in Pharmacology (2022) IF: 5.811
Tetrandrine Citrate Suppresses Breast Cancer via Depletion of Glutathione Peroxidase 4 and Activation of Nuclear Receptor Coactivator 4-Mediated Ferritinophagy

DOI: 10.3389/fphar.2022.820593

Sample: MCF7 cell,MDA-MB-231 cell
Nutrition & Metabolism (2022) IF: 4.654
Association between iron metabolism and non-alcoholic fatty liver disease: results from the National Health and Nutrition Examination Survey (NHANES 2017–2018) and a controlled animal study

DOI: 10.1186/s12986-022-00715-y

Sample: serum
Bioengineered (2021) IF: 3.269
Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axis

DOI: 10.1080/21655979.2021.2010369

PMID: 34903141
Sample: ARPE19 cell
Oncology Letters (2022) IF: 2.967
Carboxymethylated pachyman induces ferroptosis in ovarian cancer by suppressing NRF1/HO‑1 signaling

DOI: 10.3892/ol.2022.13281

Sample: SKOV3 cell,Hey cell

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K304-S
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha