Ferrous Iron Colorimetric Assay Kit
Price: $ 220
Price: $ 150
- Sample Type: serum;tissue
- Detection Instrument: Microplate reader (590-600 nm, optimum wavelength: 593 nm)
- Reasearch Areas: Inorganic ions;Plant Physiology Series;Ferroptosis
For research use only. Order now, ship in 3 days
Detection Principle | Ferrous ions (Fe2+) in samples can bind with probe to form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly. | ||||||||||||||||||
Sample Type | serum,tissue | ||||||||||||||||||
Sensitivity | 0.4 μmol/L | ||||||||||||||||||
Detection Range | 0.4-50 μmol/L | ||||||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||||||
Assay Time | 40 min | ||||||||||||||||||
Precision | inter-assay CV: 1.5% | intra-assay CV: 1.3% | ||||||||||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||||||||||
Valid Period | 12 months | ||||||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent of tissue sample is extracting solution. The diluent of serum sample is buffer solution. The serum sample has been diluted 4 times during sample processing. For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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Q1:Is the homogenate medium of E-BC-K772-M and E-BC-K773-M the same? Can a sample be treated with the same reagent to measure the total iron and ferrous content separately?
The homogenate medium is the same for both kits. Samples can be processed with the required reagents in either kit, and then tested separately for total iron and ferrous content,but be careful not to mix the other reagents in the two kits.