Ferrous Iron Colorimetric Assay Kit (E-BC-K773-M)

Name: Ferrous Iron Colorimetric Assay Kit
Brand: Elabscience
SKU: E-BC-K773-M
Price: 150.0 USD
Availability: InStock

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All	Size	Price	Qty
	96T	$ 220.00	- +
	48T	$ 150.00	- +

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Detection Principle	Ferrous ions (Fe²⁺) in samples can bind with probe to form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.
Sample Type	Serum,Tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (590-600 nm, optimum wavelength: 593 nm）
Research Area	Inorganic Ions , Plant Physiology Series , Ferroptosis
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.4 μmol/L
Detection Range	0.4-50 μmol/L
Precision	inter-assay CV: 1.5% \| intra-assay CV: 1.3%
Sample Volume	55 μL(serum/plasma), 300 μL(tissue homogenate)
Assay Time	40 min

Cat.No.	Product Name	Sizes
E-BC-K020-M	Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)	500Assays , 96T , 48T
E-BC-K025-M	Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)	500Assays , 96T , 48T
E-BC-K138-F	Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)	96T
E-BC-K772-M	Total Iron Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Mouse serum	1-2
Rat serum	1
10% Mouse liver tissue homogenate	2-3
10% Rat lung tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Rat spleen tissue homogenate	2-3
10% Epipremnum aureum leaf tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.