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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

Cat:E-BC-K025-M Citations (84)
Manual MSDS

Price: $ 220

Price: $ 150

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;animal tissue
  • Detection Instrument: Microplate reader(530-540 nm,optimum wavelength: 532 nm)
  • Reasearch Areas: Oxidative stress;Ferroptosis
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Product Details
Detection Principle MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Synonyms MDA
Sample Type serum,plasma,animal tissue
Sensitivity 1.13 μmol/L
Detection Range 2.92-40 μmol/L
Detection Method Colorimetric method
Assay Time 65 min
Precision inter-assay CV: 7.2% | intra-assay CV: 4.1%
Other Reagents Required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Images
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Human plasma 1
Rat serum 1
Rat plasma 1
Mouse serum 1
Mouse plasma 1
10% Rat heart tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat lung tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat brain tissue homogenate 1
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
  • Q1:What is the function of the control well? Is it essential?

    The control well is mainly set to eliminate the interference of the sample itself. If there is hemolysis or lipemia in the blood sample, it is necessary to set the control well; if there is no such phenomenon, it is not necessary to set the control well.

  • Q2:Can the 100 掳 C water bath mentioned in the manual be replaced by a metal bath?

    Yes, as long as the temperature can reach 100℃ and the heat is uniform.