Catalase (CAT) Activity Assay Kit (E-BC-K031-M)
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For research use only.
| Detection Principle | Catalase (CAT) can catalyze the decomposition of H2O2 into oxygen and water, and this reaction is quickly stopped upon the addition of ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm. |
| Synonyms | CAT |
| Sample Type | Serum,Plasma,Cell,Cell culture supernatant,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm, optimum wavelength: 405 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.12 U/mL |
| Detection Range | 1.12-150 U/mL |
| Precision | inter-assay CV: 7.7% | intra-assay CV: 3.9% |
| Sample Volume | 40 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| 293T supernatant | 1 |
| 10% Rat heart tissue homogenization | 50-100 |
| 10% Rat liver tissue homogenization | 100-200 |
| 10% Rat spleen tissue homogenization | 50-100 |
| Mouse serum | 1 |
| 10% Epipremnum aureum tissue homogenization | 1-2 |
| 10% Rat lung tissue homogenization | 50-100 |
| 10% Rat kidney tissue homogenization | 50-100 |
| 10% Rat brain tissue homogenization | 20-50 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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