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BCA Protein Colorimetric Assay Kit

Citations(83)

  • Cat.No.:E-BC-K318-M

  • Detection instrument: Microplate reader(540-590 nm, optimum wavelength: 562 nm)

Manual MSDS

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  • 500Assays
  • 96T
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Protocols for metabolism assays

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★: Microplate is not provided in metabolism assay kits of over 500 assays.

Detection principle

Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.

Performance characteristics

Synonyms TP
Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 0.0165 mg/mL
Detection range 0.0165-1 mg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 4.500%Average intra-assay CV: 2.200%
Other instruments required Vortex mixer, Micropipettor, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage RT
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.0165-1 mg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse brain tissue homogenization

8-12

10% Mouse kidney tissue homogenization

8-12

Human serum

100-200

10% Rat liver tissue homogenization

15-20

10% Mouse heart tissue homogenization

8-12

Rat serum

100-200

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. IMMUNITY (2023) IF: 43.474
    Pregnancy-induced changes to the gut microbiota drive macrophage pyroptosis and exacerbate septic inflammation

    DOI: 10.1016/j.immuni.2023.01.015

    PMID: 36792573

    		 Sample: BM-derived macrophages (BMDMs)
  2. CHEMICAL ENGINEERING JOURNAL (2023) IF: 16.744
    Right once for all: Zinc-modulated highly stable iron-based ROS generator under physiological conditions for promoting bacteria-infected wound healing

    DOI: 10.1016/j.cej.2023.141837
  3. SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
    Triclocarban and triclosan exacerbate high-fat diet-induced hepatic lipid accumulation at environmental related levels: The potential roles of estrogen-related receptors pathways

    DOI: 10.1016/j.scitotenv.2022.160079
  4. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
    BMSC exosome-enriched acellular fish scale scaffolds promote bone regeneration

    DOI: 10.1186/s12951-022-01646-9

    PMID: 36224596

    		 Sample: BMSCs
  5. BRAIN BEHAVIOR AND IMMUNITY (2021) IF: 7.217
    Acute neuroinflammation increases excitability of prefrontal parvalbumin interneurons and their functional recruitment during novel object recognition

    DOI: 10.1016/j.bbi.2021.08.216

    PMID: 34403738

    		 Sample: medial prefrontal cortex (mPFC)
  6. Materials Today Bio (2021) IF: 7.348
    Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer

    DOI: 10.1016/j.mtbio.2021.100189
    Sample: RM-1 cell,LNCaP PCa cell
  7. JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.446
    MPLA-Adjuvanted Liposomes Encapsulating S-Trimer or RBD or S1, but Not S-ECD, Elicit Robust Neutralization Against SARS-CoV-2 and Variants of Concern

    DOI: 10.1021/acs.jmedchem.1c02025

    PMID: 35108485

    		 Sample: liposome
  8. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo

    DOI: 10.3390/ijms232416134
    Sample: Lung tissue,THP-1 cells
  9. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats

    DOI: 10.3390/ijms231810920

    PMID: 36142831

    		 Sample: muscle
  10. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
    Low trifloxystrobin-tebuconazole concentrations induce cardiac and developmental toxicity in zebrafish by regulating notch mediated‐oxidative stress generation

    DOI: 10.1016/j.ecoenv.2022.113752
    Sample: embryo
  11. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Carveol Attenuates Seizure Severity and Neuroinflammation in Pentylenetetrazole-Kindled Epileptic Rats by Regulating the Nrf2 Signaling Pathway

    DOI: 10.1155/2021/9966663

    PMID: 34422216

    		 Sample: brain
  12. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2023) IF: 6.633
    A prospective diagnostic and prognostic biomarker for hepatocellular carcinoma that functions in glucose metabolism regulation: Solute carrier family 37 member 3

    DOI: 10.1016/j.bbadis.2023.166661
    Sample: HL7702 cell,HepG2 cell,HuH-7 cell
  13. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2022) IF: 5
    m6A methyltransferase METTL3 participated in sympathetic neural remodeling post-MI via the TRAF6/NF-κB pathway and ROS production

    DOI: 10.1016/j.yjmcc.2022.06.004

    PMID: 35717715

    		 Sample: cardiac tissue
  14. EUROPEAN JOURNAL OF PHARMACOLOGY (2023) IF: 5.195
    Pentoxifylline treats Aspergillus fumigatus keratitis by reducing fungal burden and suppressing corneal inflammation

    DOI: 10.1016/j.ejphar.2023.175607
    Sample: corneas,RAW 264.7 cell
  15. Frontiers in Chemistry (2022) IF: 5.221
    Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells

    DOI: 10.3389/fchem.2022.863364

    PMID: 35464198

    		 Sample: GMSCs
  16. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
    New insights into the central sympathetic hyperactivity post-myocardial infarction: Roles of METTL3-mediated m6A methylation

    DOI: 10.1111/jcmm.17183
    Sample: brain tissue
  17. Food Bioscience (2022) IF: 5.318
    Phytosterol of lotus seed core powder alleviates hypercholesterolemia by regulating gut microbiota in high-cholesterol diet-induced C57BL/6J mice

    DOI: 10.1016/j.fbio.2022.102279
    Sample: serum,liver tissue,fecal lipids
  18. Food & Function (2022) IF: 5.396
    Screening and identification of a novel antidiabetic peptide from collagen hydrolysates of Chinese giant salamander skin: network pharmacology, inhibition kinetics and protection of IR-HepG2 cells

    DOI: 10.1039/D1FO03527D

    PMID: 35260876

    		 Sample: HepG2 cell
  19. JOURNAL OF DERMATOLOGICAL SCIENCE (2022) IF: 5.408
    Sestrin2 Contributes to BRAF Inhibitor Resistance Via Reducing Redox Vulnerability of Melanoma Cells

    DOI: 10.1016/j.jdermsci.2022.12.007
    Sample: 451Lu cells,UACC62 cells
  20. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 5.5
    microRNA-15 Activates NF-κB Pathway via Down Regulating Expression of Adenosine A2 Receptor in Ulcerative Colitis

    DOI: 10.1159/000495718
    Sample: colonic tissues,HT-29 cells
  21. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Mechanism of antifungal activity and therapeutic action of β-ionone on Aspergillus fumigatus keratitis via suppressing LOX1 and JNK/p38 MAPK activation

    DOI: 10.1016/j.intimp.2022.108992

    PMID: 35810488

    		 Sample: corneas
  22. INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
    Dimethyl fumarate ameliorates fungal keratitis by limiting fungal growth and inhibiting pyroptosis

    DOI: 10.1016/j.intimp.2023.109721
    Sample: corneal tissue,Human corneal epithelial cells (HCE
  23. Stem Cell Reviews and Reports (2022) IF: 5.739
    Adipose Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing Through the WNT/β-catenin Signaling Pathway in Dermal Fibroblasts

    DOI: 10.1007/s12015-022-10378-0

    PMID: 35471485

    		 Sample: ADMSC-Exos
  24. Frontiers in Plant Science (2022) IF: 5.754
    MC03g0810, an Important Candidate Gene Controlling Black Seed Coat Color in Bitter Gourd (Momordica spp.).

    DOI: 10.3389/fpls.2022.875631
    Sample: seed coat
  25. Frontiers in Pharmacology (2022) IF: 5.811
    Tilianin Reduces Apoptosis via the ERK/EGR1/BCL2L1 Pathway in Ischemia/Reperfusion-Induced Acute Kidney Injury Mice.

    DOI: 10.3389/fphar.2022.862584
    Sample: kidney tissue
  26. Pharmaceuticals (2021) IF: 5.863
    Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male Mice

    DOI: 10.3390/ph15010016
    Sample: serum
  27. Frontiers in Pharmacology (2022) IF: 5.988
    Ferredoxin 1 is a cuproptosis-key gene responsible for tumor immunity and drug sensitivity: A pan-cancer analysis.

    DOI: 10.3389/fphar.2022.938134
    Sample: BLCA cell,ccRCC cell,PCa cell
  28. Biomolecules (2020) IF: 4.082
    Post-Treatment of Synthetic Polyphenolic 1,3,4 Oxadiazole Compound A3, Attenuated Ischemic Stroke-Induced Neuroinflammation and Neurodegeneration

    DOI: 10.3390/biom10060816

    PMID: 32466476

    		 Sample: Tissue homogenate
  29. ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119
    Compound essential oils relieve oxidative stress caused by PM2.5 exposure by inhibiting autophagy through the AMPK/mTOR pathway

    DOI: 10.1002/tox.23297
    Sample: Cell culture supernatant
  30. BIOLOGY OF REPRODUCTION (2023) IF: 4.161
    Extracellular vesicles contribute to EMT in Adenomyosis by inducing macrophage polarization

    DOI: 10.1093/biolre/ioad015
    Sample: primary endometrial cell
  31. Human Cell (2022) IF: 4.174
    USP36 promotes tumor growth of non-small cell lung cancer via increasing KHK-A expression by regulating c-MYC–hnRNPH1/H2 axis

    DOI: 10.1007/s13577-022-00677-6
    Sample: A549 cell,PC-9 cell,NCI-H292 cell,NCI-1975 cell,BE
  32. GENOMICS (2022) IF: 4.31
    CircEML1 facilitates the steroid synthesis in follicular granulosa cells of chicken through sponging gga-miR-449a to release IGF2BP3 expression

    DOI: 10.1016/j.ygeno.2022.110540
    Sample: granulosa cells (GCs)
  33. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Revealing the mechanism of “Huai Hua San” in the treatment of ulcerative colitis based on network pharmacology and experimental study

    DOI: 10.1016/j.jep.2021.114321
  34. ACS Chemical Neuroscience (2021) IF: 4.418
    Synthesis and Biological Evaluation of Benzimidazole Derivatives as Potential Neuroprotective Agents in an Ethanol-Induced Rodent Model

    DOI: 10.1021/acschemneuro.0c00659

    PMID: 33430586

    		 Sample: Tissue homogenate(Brain)
  35. JOURNAL OF PERIODONTAL RESEARCH (2022) IF: 4.419
    Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells

    DOI: 10.1111/jre.12973

    PMID: 35037251

    		 Sample: Periodontal ligament stem cell (PDLSCs)
  36. Frontiers in Physiology (2021) IF: 4.566
    Sleeve Gastrectomy Ameliorates Diabetes-Induced Cardiac Hypertrophy Correlates With the MAPK Signaling Pathway

    DOI: 10.3389/fphys.2021.785799

    PMID: 34858216

    		 Sample: heart
  37. Frontiers in Neuroscience (2022) IF: 4.677
    Neuroprotective Effect of Otostegia limbata Against PTZ-Induced Mice Model of Epilepsy by Attenuated Expression of p-NFκB and TNF-α

    DOI: 10.3389/fnins.2022.779681

    PMID: 35392411

    		 Sample: cortex,hippocampus
  38. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2022) IF: 4.925
    Gallic Acid Ameliorates Aspergillus Fumigatus Keratitis Through Reducing Fungal Load and Suppressing the Inflammatory Response

    DOI: 10.1167/iovs.63.12.12
  39. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Quercetin ameliorates Aspergillus fumigatus keratitis by inhibiting fungal growth, toll-like receptors and inflammatory cytokines

    DOI: 10.1016/j.intimp.2021.107435
    Sample: Tissue homogenate(corneas)
  40. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Pravastatin attenuates sepsis-induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of Cav-1/eNOS pathway

    DOI: 10.1016/j.intimp.2021.108077

    PMID: 34464887

    		 Sample: BALF
  41. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Isoliquiritigenin alleviates P. gingivalis-LPS/ATP-induced pyroptosis by inhibiting NF-κB/ NLRP3/GSDMD signals in human gingival fibroblasts

    DOI: 10.1016/j.intimp.2021.108338

    PMID: 34794890
  42. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    High-density lipoprotein reduces microglia activation and protects against experimental autoimmune encephalomyelitis in mice

    DOI: 10.1016/j.intimp.2022.108566

    PMID: 35101849

    		 Sample: spinal cord,BV2 microglial cell
  43. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Effect of gingival mesenchymal stem cell-derived exosomes on inflammatory macrophages in a high-lipid microenvironment

    DOI: 10.1016/j.intimp.2021.107455

    PMID: 33582592

    		 Sample: Cell culture supernatant
  44. LWT-FOOD SCIENCE AND TECHNOLOGY (2021) IF: 4.952
    Bio-electrochemical impedance analysis of frozen Japanese pear tissues: And the relationships among the physical properties, total polyphenol content, and oxidase activity

    DOI: 10.1016/j.lwt.2021.112499
    Sample: fruit
  45. EUROPEAN JOURNAL OF MEDICAL RESEARCH (2022) IF: 4.981
    Inhibition of p38 mitogen-activated protein kinases may attenuate scar proliferation after cleft lip surgery in rabbits via Smads signaling pathway

    DOI: 10.1186/s40001-022-00757-1

    PMID: 35858881

    		 Sample: Fibroblast
  46. Journal of Food Measurement and Characterization (2022) IF: 3.006
    Isolation and structural characterization of antioxidant peptides from horse bone marrow protein hydrolysates

    DOI: 10.1007/s11694-022-01638-1
    Sample: bone marrow
  47. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    A relationship between MAPK/ERK pathway expression and neuronal apoptosis in rats with white matter lesions

    DOI: 10.26355/eurrev_202004_21023

    PMID: 32373979

    		 Sample: Tissue homogenate
  48. Journal of Integrative Medicine-JIM (2022) IF: 3.034
    Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway

    DOI: 10.1016/j.joim.2022.01.007

    PMID: 35153135

    		 Sample: ovarian tissue
  49. Frontiers in Bioscience-Landmark (2022) IF: 3.115
    SIRT3 Enhances the Protective Role of Propofol in Postoperative Cognitive Dysfunction via Activating Autophagy Mediated by AMPK/mTOR Pathway

    DOI: 10.31083/j.fbl2711303
    Sample: hippocampal tissue,Primary Neurons
  50. EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
    The antidepressant effects of hesperidin on chronic unpredictable mild stress-induced mice

    DOI: 10.1016/j.ejphar.2019.03.035

    PMID: 30928632

    		 Sample: Cell culture supernatant,Tissue homogenate
  51. Frontiers in Physiology (2019) IF: 3.201
    Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

    DOI: 10.3389/fphys.2019.01430

    PMID: 31824338

    		 Sample: Plasma
  52. PLoS One (2022) IF: 3.24
    Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats

    DOI: 10.1371/journal.pone.0266898

    PMID: 35421180

    		 Sample: Brain tissue
  53. MOLECULES (2020) IF: 3.267
    The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury

    DOI: 10.3390/molecules25204612

    PMID: 33050458

    		 Sample: Cell lysate
  54. Bioengineered (2022) IF: 3.269
    Tanshinone IIA alleviates cardiac hypertrophy through m6A modification of galectin-3

    DOI: 10.1080/21655979.2022.2031388

    PMID: 35191812
  55. Bioengineered (2021) IF: 3.269
    Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axis

    DOI: 10.1080/21655979.2021.2010369

    PMID: 34903141

    		 Sample: ARPE19 cell
  56. Diabetology & Metabolic Syndrome (2022) IF: 3.32
    LncRNA HOTAIR facilitates high glucose-induced mesangial cell proliferation, fibrosis and oxidative stress in diabetic nephropathy via regulating miR-147a/WNT2B axis

    DOI: 10.1186/s13098-022-00802-3

    PMID: 35193668
  57. JOURNAL OF PHARMACOLOGICAL SCIENCES (2021) IF: 3.337
    Sesamin promotes apoptosis and pyroptosis via autophagy to enhance antitumour effects on murine T-cell lymphoma

    DOI: 10.1016/j.jphs.2021.08.001

    PMID: 34507635

    		 Sample: EL4 cell
  58. POULTRY SCIENCE (2021) IF: 3.352
    Adiponectin modulates steroid hormone secretion, granulosa cell proliferation and apoptosis via binding its receptors during hens’ high laying period

    DOI: 10.1016/j.psj.2021.101197
    Sample: Tissue homogenate
  59. Brain and Behavior (2022) IF: 3.405
    The protective effects of nesfatin-1 in neurological dysfunction after spinal cord injury by inhibiting neuroinflammation

    DOI: 10.1002/brb3.2778

    PMID: 36271663
  60. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS (2023) IF: 3.405
    Copper-deposited diatom-biosilica enhanced osteogenic potential in periodontal ligament stem cells and rat cranium

    DOI: 10.1002/jbm.b.35232
    Sample: hPDLSCs
  61. Frontiers in Veterinary Science (2022) IF: 3.412
    Rutin Supplementation Reduces Oxidative Stress, Inflammation and Apoptosis of Mammary Gland in Sheep During the Transition Period

    DOI: 10.3389/fvets.2022.907299
    Sample: mammary gland tissue
  62. Canadian Journal of Gastroenterology and Hepatology (2022) IF: 3.522
    LncRNA PVT1 Promotes Cell Proliferation, Invasion, and Migration and Inhibits Cell Apoptosis by Phosphorylating YAP

    DOI: 10.1155/2022/5332129

    PMID: 35664988

    		 Sample: MKN-45 cell
  63. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2021) IF: 3.575
    Downregulation of circular RNA circDOCK7 identified from diabetic rats after sleeve gastrectomy contributes to hepatocyte apoptosis through regulating miR-139–3p and MCM3

    DOI: 10.1016/j.bbrc.2021.02.069
    Sample: Cell lysate(BRL cell lines (normal rat hepatocytes
  64. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Network pharmacology, molecular docking and in vivo and in vitro experiments to explore the molecular mechanism of licorice green tea beverage to scavenge oxygen free radicals

    DOI: 10.1111/jfbc.14315

    PMID: 35855584

    		 Sample: Hacat cell
  65. Frontiers in Neuroscience (2020) IF: 3.707
    Potent Natural Antioxidant Carveol Attenuates MCAO-Stress Induced Oxidative, Neurodegeneration by Regulating the Nrf-2 Pathway

    DOI: 10.3389/fnins.2020.00659
    Sample: Tissue homogenate
  66. BRAIN RESEARCH BULLETIN (2022) IF: 3.715
    Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke

    DOI: 10.1016/j.brainresbull.2022.09.015

    PMID: 36152772

    		 Sample: brain tissue
  67. Heliyon (2023) IF: 3.776
    Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions

    DOI: 10.1016/j.heliyon.2023.e12958
    Sample: HTR-8/SVneo cell
  68. MICROBIAL PATHOGENESIS (2022) IF: 3.848
    Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro

    DOI: 10.1016/j.micpath.2022.105964
    Sample: bone marrow-derived macrophages (BMMs)
  69. EXPERIMENTAL CELL RESEARCH (2022) IF: 3.905
    p38MAPK silencing attenuates scar proliferation after cleft palate repair surgery in rats via MRTF-A/SRF pathway

    DOI: 10.1016/j.yexcr.2022.113248

    PMID: 35690133

    		 Sample: fibroblast
  70. Annals of Translational Medicine (2022) IF: 3.932
    SIRT3-mediated mitochondrial autophagy in refeeding syndrome-related myocardial injury in sepsis rats

    DOI: 10.21037/atm-22-222

    PMID: 35280405

    		 Sample: cardioid
  71. Annals of Translational Medicine (2022) IF: 3.932
    Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

    DOI: 10.21037/atm-22-483

    PMID: 35280364

    		 Sample: myocardium
  72. NEUROSCIENCE LETTERS (2018) IF: 2.159
    Regular aerobic exercise correlates with reduced anxiety and incresed levels of irisin in brain and white adipose tissue

    DOI: 10.1016/j.neulet.2018.04.023
    Sample: Serum,Plasma,Whole Blood,Platelet,Tissue homogenat
  73. ARCHIVES OF GYNECOLOGY AND OBSTETRICS (2022) IF: 2.344
    Effect of Neiyi Prescription of QIU on autophagy and angiogenic ability of endometriosis via the PPARγ/NF-κB signaling pathway

    DOI: 10.1007/s00404-022-06537-w

    PMID: 35366690

    		 Sample: ectopic endometria
  74. ENDOCRINE JOURNAL (2022) IF: 2.349
    Human umbilical cord mesenchymal stem cells alleviate the imbalance of CD4+ T cells via protein tyrosine phosphatase non-receptor type 2/signal transducer and activator of transcription 3 signaling in ameliorating experimental autoimmune thyroiditis in ra

    DOI: 10.1507/endocrj.EJ20-0695
    Sample: CD4+ T cell
  75. BIOLOGICAL TRACE ELEMENT RESEARCH (2018) IF: 2.361
    Timeline (Bioavailability) of Magnesium Compounds in Hours: Which Magnesium Compound Works Best?

    DOI: 10.1007/s12011-018-1351-9
    Sample: brain tissues,plasma,muscle tissues
  76. Experimental and Therapeutic Medicine (2022) IF: 2.447
    Salvianolic acid A promotes mitochondrial biogenesis and function via regulating the AMPK/PGC‑1α signaling pathway in HUVECs

    DOI: 10.3892/etm.2022.11412
    Sample: Primary HUVECs
  77. ANDROLOGIA (2021) IF: 2.775
    Runjing Decoction alleviated cyclophosphamide-induced oligoasthenospermia rats by inhibiting cell apoptosis via RXFP1/AKT/FOXO1 pathway

    DOI: 10.1111/and.14216

    PMID: 34396564

    		 Sample: testis tissue
  78. Journal of Pain Research (2023) IF: 2.832
    The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study

    DOI: 10.2147/JPR.S400871
    Sample: dorsal root ganglion (DRG) tissue,intervertebral d
  79. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2019) IF: 2.914
    Nicotine increased VEGF and MMP2 levels in the rat eye and kidney

    DOI: 10.1007/s11356-019-06460-9
  80. JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2022) IF: 2.945
    CircCDC6 restrains tumor growth and glycolysis energy metabolism in colorectal cancer via regulating miR-3187-3p and downstream PRKAA2

    DOI: 10.1007/s10863-022-09938-3

    PMID: 35438362

    		 Sample: SW480 cell,HCT116 cell
  81. GENE (2020) IF: 2.984
    Chitosan-stabilized selenium nanoparticles alleviate cardio-hepatic damage in type 2 diabetes mellitus model via regulation of caspase, Bax/Bcl-2, and Fas/FasL-pathway

    DOI: 10.1016/j.gene.2020.145288

    PMID: 33181259

    		 Sample: Serum
  82. Brazilian Journal of Cardiovascular Surgery (2020) IF: 1.053
    Effect of Shenfu Injection on Isolated Empty Beating Hearts from Miniature Pigs

    DOI: 10.21470/1678-9741-2019-0264
    Sample: Tissue homogenate
  83. NEUROREPORT (2021) IF: 1.837
    Protective effects of melatonin on the white matter damage of neonatal rats by regulating NLRP3 inflammasome activity

    DOI: 10.1097/WNR.0000000000001642

    PMID: 33994520

    		 Sample: periventricular
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Reviews/Q&A

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  • Q&A
Q

T****************nSubmitted [ Jan 10 2024 ]

Asked: can this kit use to measure protein in zebrafish tissue samples,tq

Reply

A

adminSubmitted [ Jan 10 2024 ]

Answered: This kit can be used to measure tissue samples regardless of species. Our laboratory has not verified the zebrafish tissue samples, but theoretically, if the protein content of the samples is higher than the sensitivity of the kit, it can be detected.

Q

aSubmitted [ Dec 08 2023 ]

Asked: can this be used to assess stool protein level

Reply

Q

M***********dSubmitted [ Jun 11 2023 ]

Asked: Can this kit be used for bronchoalveolar lavage samples? And what should be the dilution factor of mouse lung tissue samples

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A

adminSubmitted [ Jun 11 2023 ]

Answered: Hello Muntaha javid,thanks for your support.Our kit is suitable for bronchoalveolar lavage samples.Lung tissue sample processing: 10% sample homogenate diluted 10 times.

Q

M*****aSubmitted [ Aug 01 2022 ]

Asked: E-BC-K318-M is ok for use with Human salive samples. If so, can you please send me salive samples method ?

Reply

A

adminSubmitted [ Aug 01 2022 ]

Answered: Saliva: gargling with clear water, after 30 min saliva was collected and centrifuged at 10000 g at 4℃ for 5 min. Then take the supernatant for detection. If not detected on the same day, the saliva can be stored at-80℃ for a month.

Q

M*****aSubmitted [ Aug 01 2022 ]

Asked: How long is the shelf life of this kit

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A

adminSubmitted [ Aug 01 2022 ]

Answered:  The shelf life of this kit is 12 months.

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