(KO Validated) POR Polyclonal Antibody
- +4
Price: $ 580
Price: $ 360
Price: $ 220
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:HeLa,A549,HeLa,MCF-7,293T,Mouse liver,Mouse kidney Verified Samples in IHC:Human esophagus Verified Samples in IF:C6, HeLa, NIH/3T3 |
Dilution |
WB 1:500-1:2000, IHC 1:50-1:100, IF 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant fusion protein of human POR (NP_000932.3). |
Abbre | POR |
Synonyms | POR;CPR;CYPOR;P450R |
Swissprot | |
Calculated MW | 76kDa |
Observed MW |
77kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Endoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region. |
Concentration | 1mg/mL |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Cardiovascular; Metabolism; Signal Transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. |