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ABCB5 Monoclonal Antibody

Cat:E-AB-22032
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human
  • Applications: WB;IHC-p;IF
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Hela,293T
Verified Samples in IHC:Human breast cancer
Verified Samples in IF:Human liver cancer
Dilution

WB 1:500-1:3000, IHC 1:50-1:300, IF 1:100-1:300

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Synthetic Peptide
Abbre ABCB5
Synonyms ABCB 5;ABCB5;ABCB5 P-gp;ABCB5;ABCB5alpha;ABCB5beta;ATP binding cassette protein;ATP binding cassette sub family B (MDR/TAP) member 5;ATP binding cassette sub family B member 5;ATP-binding cassette sub-family B member 5;EST422562;P glycoprotein ABCB5;P-glycoprotein ABCB5
Swissprot
Calculated MW 90kDa
Observed MW 90kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell membrane.
Tissue Specificity Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method Protein A purification
Research Areas Cancer; Metabolism; Signal Transduction
Clone No. Clone:7L3
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.