Acetylcholinesterase (AchE) Activity Assay Kit
Price: $ 220
Price: $ 150
Size:
96T
48T
Quantity:
- Sample Type: serum;plasma;animal tissue;cell
- Detection Instrument: Microplate reader(optimum wavelength: 412 nm)
- Reasearch Areas: Amino acids proteins
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Product Details
Detection Principle | AchE catalyzes the hydrolysis of acetylcholine to form choline, and choline react with dithio p-nitrobenzoic acid (DTNB) to form 5-mercapto-nitrobenzoic acid (TNB). TNB has an absorption peak at 412nm. And the activity of AchE is calculated by measuring the increasing rate of absorbance at 412nm. | ||||||||||||||||||||||||
Synonyms | AchE | ||||||||||||||||||||||||
Sample Type | serum,plasma,animal tissue,cell | ||||||||||||||||||||||||
Sensitivity | 1.225 U/mL | ||||||||||||||||||||||||
Detection Range | 1.225-490 U/mL | ||||||||||||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||||||||||||
Assay Time | 20 min | ||||||||||||||||||||||||
Precision | inter-assay CV: 9.3% | intra-assay CV: 4.7% | ||||||||||||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) | ||||||||||||||||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||||||||||||||||
Valid Period | 12 months | ||||||||||||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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