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Acetylcholinesterase (AchE) Activity Assay Kit

Cat:E-BC-K174-M Citations (6)
Manual MSDS

Price: $ 220

Price: $ 150

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;animal tissue;cell
  • Detection Instrument: Microplate reader(optimum wavelength: 412 nm)
  • Reasearch Areas: Amino acids proteins
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Product Details
Detection Principle AchE catalyzes the hydrolysis of acetylcholine to form choline, and choline react with dithio p-nitrobenzoic acid (DTNB) to form 5-mercapto-nitrobenzoic acid (TNB). TNB has an absorption peak at 412nm. And the activity of AchE is calculated by measuring the increasing rate of absorbance at 412nm.
Synonyms AchE
Sample Type serum,plasma,animal tissue,cell
Sensitivity 1.225 U/mL
Detection Range 1.225-490 U/mL
Detection Method Colorimetric method
Assay Time 20 min
Precision inter-assay CV: 9.3% | intra-assay CV: 4.7%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Mouse serum 8-20
Mouse plasma 4-10
Human serum 4-10
Human plasma 4-10
Rat serum 4-10
Dog serum 4-10
Horse serum 2-8
10% Mouse liver tissue homogenate 1
10% Mouse kidney tissue homogenate 1
10% Mouse brain tissue homogenate 2-8
10% Crucian carp muscle tissue homogenate 1
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.