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Adenosine Deaminase (ADA) Activity Assay Kit

Cat.No.:E-BC-K197-M
Detection instrument: Microplate reader (550 nm)

Detection method: Colorimetric method

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Detection principle

Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA.

Performance characteristics

Synonyms	ADA
Sample type	serum, plasma, animal tissue
Sensitivity	0.03 U/L
Detection range	0.03-99 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	40 min
Precision	Average inter-assay CV: 6%Average intra-assay CV: 3%
Other instruments required	Micropipette, Centrifuge, Water bath/Incubator
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-99 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Mouse liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat heart tissue homogenate	2-3
10% Rat spleen tissue homogenate	2-3
Mouse serum	1
Human serum	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Biomaterials Science (2022) IF: 7.59
Transdermal delivery of allopurinol on acute hyperuricemic mice via polymer microneedles for regulation of serum uric acid levels

DOI: 10.1039/D2BM01836E

Sample: liver tissue
Heliyon (2021)
Treatment with acetate during late pregnancy protects dams against testosterone-induced renal dysfunction

DOI: 10.1016/j.heliyon.2021.e05920

Sample: Renal

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