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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (E-BC-K235-M)

All Size Price Qty
96T $ 180.00
48T $ 120.00
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For research use only.

Detection Principle ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Synonyms ALT;GPT;Glutamic-pyruvic transaminase
Sample Type Serum,Plasma,Animal tissue,Cell,Cell culture supernatant
Detection Method Colorimetric method
Detection Instrument Microplate reader (500-520 nm)
Research Area Liver And Renal Function
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.75 IU/L
Detection Range 0.75-72.30 IU/L
Precision inter-assay CV: 0.09 | intra-assay CV: 5.3%
Assay Time 1 h 20 min
Cat.No. Product Name Sizes
E-BC-K236-M Aspartate Aminotransferase (AST/GOT) Activity Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Human plasma 1
Porcine serum 1
Rat serum 1
10% Rat brain tissue homogenization 1
10% Rat heart tissue homogenization 1
10% Rat liver tissue homogenization 40-60
10% Rat kidney tissue homogenization 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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