Adenosine Diphosphate (ADP) Fluorometric Assay Kit (E-BC-F009)
For research use only.
| Detection Principle |
Adenosine diphosphate (ADP) is a nucleoside that plays a key role in energy transfer reactions. In organisms, ADP is usually the product of Adenosine triphosphate (ATP) which hydrolysis after the loss of a phosphate group, that is breaking a high-energy phosphate bond and releasing energy. ADP exists in high-density particles in platelet cells and is released when platelets undergo coagulation reaction. ADP affects the shape and biological behavior of platelets through ADP receptors on platelets, and further accelerates the process of platelet coagulation. The detection principle of this kit is that ADP and the substrate are catalyzed by enzyme reagents to make the chromogenic agent produce fluorescence. The fluorescence microplate reader was used to detect the excitation wavelength of 535 nm and the emission wavelength of 587nm, and the ADP content in the sample was calculated according to the standard curve. |
| Synonyms | ADP |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Metabolic Diseases , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.8 μmol/L |
| Detection Range | 2.8-50 μmol/L |
| Precision | inter-assay CV: 4.4-9.6% | intra-assay CV: 4.0-5.0% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1-3 |
| 10% Rat kidney tissue homogenate | 1-3 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1-3 |
| Mouse plasma | 1-3 |
| Rat serum | 1 |
| 1 × 10^6 Molt-4 cell | 1 |
| 1.2 × 10^6 HL-60 cell | 1 |
| 1 × 10^6 293T cell | 1 |
| 1.3 × 10^6 CHO cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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