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Adipogenesis Fluorometric Assay Kit

Cat.No.:E-BC-F065
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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96T

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Detection principle

Triglycerides were converted by enzyme to produce hydrolytic products, which were catalyzed by the enzyme to produce fluorescent substances. The triglyceride accumulation can be calculated by meas-uring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.

Performance characteristics

Sample type	cell
Sensitivity	1.4 μmol/L
Detection range	1.4-32 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 8.400%Average intra-assay CV: 4%
Other instruments required	Micropipettor, Incubator, Centrifuge
Storage	Reagent 3: -20℃; Others: 2-8℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-F041	Glucose Uptake Fluorometric Assay Kit
E-BC-F039	Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

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Collection and treatment of common samples for metabolism assay detection

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