Purified Anti-Human CD19 Antibody[HIB19], Functional Grade (AN008610)
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For research use only.
| Alternate Names | CVID, B-lymphocyteantigenCD, B-lymphocyte antigen CD, T-cellsurfaceantigenLeu, Differentiation antigen CD, T-cell surface antigen Leu, B-lymphocytesurfaceantigenB, B-lymphocyte surface antigen B, B4, CVID3, CD19, B-lymphocyteantigenCD19, B-lymphocytesurfaceantigenB4, T-cellsurfaceantigenLeu-12, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, B4, CVID3, CD19, CD19 molecule |
| Clone | |
| Leadtime | Inquire |
| Verified Samples | Verified Samples in FCM: Human peripheral blood lymphocytes cell |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Block Reported in the literaturee |
| Background | CD19 protein is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses . CD19 is expressed throughout B cell development from pre-B cells through mature B cells, and it is commonly used as a B cell lineage marker . It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity matuRation . The CD19 protein associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13. These associations enable CD19 to amplify B cell signaling and reduce the threshold for antigen stimulation through the BCR. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Human CD19 protein |
| Abbre | CD19 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG1, κ |
| Isotype Control | Purified Mouse IgG1, κ Isotype Control[MOPC-21], Functional Grade |
| Applications |
FCM;Block
|
| Research Areas | Immunology;Stem Cells |
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Walker JD, et al. J. Immunol. 2009;182:1548. |
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