Purified Anti-Human HLA-A,B,C Antibody[W6/32], Functional Grade (E-AB-F11300)
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For research use only.
| Alternate Names | MHC class I, Major Histocompatibility Class I |
| Clone | |
| Leadtime | Order now, ship in 3 days |
| Verified Samples | Verified Samples in FCM: Human peripheral blood lymphocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);FA Reported in the literature |
| Background | MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant MHC class I protein |
| Abbre | HLA-A,B,C |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG2a, κ |
| Isotype Control | Purified Mouse IgG2a, κ Isotype Control[C1.18.4], Functional Grade |
| Applications |
FCM;FA
|
| Research Areas | Immunology;Innate Immunity |
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | N M Valenzuela, et al. Am J Transplant. 2013 Feb;13(2):299-311. |
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