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Elab Fluor® 488 Anti-Human CD44 Antibody[Hermes-1]

Uniprot : P16070
  • Cat.No.:E-AB-F1215L

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1215L

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  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $70
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Antigen Details

Alternate Names LHR,PGP-I,CDw44,Epican,HUTCH-I
Swissprot P16070
References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.

Product Details

Form Liquid
Clone No. Hermes-1(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control Elab Fluor® 488 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832L]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Elab Fluor® 488
Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Human CD44 Antibody[Hermes-1] 50μg, 500μg, 1mg Check More
Purified Anti-Human CD44 Antibody[Hermes-1] 25μg, 100μg Check More
FITC Anti-Human CD44 Antibody[Hermes-1] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD44 Antibody[Hermes-1] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD44 Antibody[Hermes-1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD44 Antibody[Hermes-1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Human CD44 Antibody[Hermes-1] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD44 Antibody[Hermes-1] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Human CD44 Antibody[Hermes-1] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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