Current Position:Home>>Antibodies>>FCM Antibodies>> AF647 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7] E-AB-F1191UM
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Add to cart Bulk requestAlternate Names | Ly6a,Ly6,Ly-6A.2/Ly-6E.1,SCA-1,TAP |
Swissprot | P05533 |
References |
1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709. |
Form | Liquid |
Clone No. | D7(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | AF647 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833M] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | PBS with 0.05% Proclin300, 1% BSA |
Recommended Use | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | AF647 |
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Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |