Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Human CD11a Antibody[R7-1]
Cat.No.:E-AB-F12110
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Alternate Names | Integrin alpha-L,LFA-1A,CD11A,ITGAL |
Swissprot | P20701 |
References |
1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146. |
Form | Liquid |
Clone No. | R7-1(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | AF/LE Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F097930] |
Reactivity | Human |
Application | FA,FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
Purified Anti-Human CD11a Antibody[R7-1] | 25μg, 100μg | Check More |
FITC Anti-Human CD11a Antibody[R7-1] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human CD11a Antibody[R7-1] | 20Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Human CD11a Antibody[R7-1] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Human CD11a Antibody[R7-1] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Human CD11a Antibody[R7-1] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 647 Anti-Human CD11a Antibody[R7-1] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Elab Fluor® 594 Anti-Human CD11a Antibody[R7-1] | 20Tests, 100Tests, 100Tests×2 | Check More |