Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F11330
Cat.No.:E-AB-F11330
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Alternate Names | B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1 |
Swissprot | Q9NZQ7 |
References |
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
|
Form | Liquid |
Clone No. | 29E.2A3(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG2b, κ |
Isotype Control | AF/LE Purified Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F098130] |
Reactivity | Human |
Application | Block,FA,FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133A | 25μg, 100μg | Check More |
Biotin Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133B | 25μg, 100μg | Check More |
FITC Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133C | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133D | 20Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133E | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133G | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133H | 20Tests, 100Tests, 100Tests×2 | Check More |
PerCP/Cyanine5.5 Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133J | 20Tests, 100Tests, 100Tests×2 | Check More |
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