Alternate Names	T-cell surface glycoprotein CD4,CD4,T-cell surface antigen T4/Leu-3,CD4
Swissprot	P01730
References	1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557.

Product Details

Form	Liquid
Clone No.	RPA-T4(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	AF/LE Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F097930]
Reactivity	Human
Application	Block,FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human CD4 Antibody[RPA-T4]	25μg, 100μg	Check More
Biotin Anti-Human CD4 Antibody[RPA-T4]	25μg, 100μg	Check More
FITC Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human CD4 Antibody[RPA-T4]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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