Alternate Names	T-cell surface glycoprotein CD8 alpha chain,CD8A,T-lymphocyte differentiation antigen T8/Leu-2,MAL
Swissprot	P01732
References	1. Knapp W. W. Knapp .. et al.Oxford University Press; 1989; :1-1182. 2. Reimann KA, Waite BC, Lee-Parritz DE, et al. Cytometry. 1994; 17(1):102-108. 3. Roederer M, Kantor AB, Parks DR, Herzenberg LA. Cytometry. 1996; 24(3):191-197. 4. Schlossman SF. Stuart F. Schlossman .. et al. Oxford University Press; 1995. 5. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Cytometry. 1997; 29(4):351-362.

Product Details

Form	Liquid
Clone No.	OKT-8(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a, κ
Isotype Control	AF/LE Purified Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F098030]
Reactivity	Human
Application	Depletion,FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human CD8a Antibody[OKT-8]	25μg, 100μg	Check More
Biotin Anti-Human CD8a Antibody[OKT-8]	25μg, 100μg	Check More
FITC Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human CD8a Antibody[OKT-8]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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