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Cat.No.:E-AB-F11110
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Alternate Names | HLA class II histocompatibility antigen DR alpha/ DRB1-15 beta chain,MHC class II antigen DRA,HLA-DRA1/DRB1,DRA/DRB1 |
Swissprot | P01903,P01911 |
References |
1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leuk. Biol. 48:138. 3. Edwards J, et al. 1985. J. Immunol. 137:490. 4. van Es A, et al. 1984. Transplantation 37:65. 5. O'Doherty U, et al. 1994. Immunology 82:487. 6. Thomas R, et al. 1994. J. Immunol. 153:4016. 7. Grouard G, et al. 1996. Nature 384:364. |
Form | Liquid |
Clone No. | L243(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG2a, κ |
Isotype Control | AF/LE Purified Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F098030] |
Reactivity | Human |
Application | Cell Sep-Neg,Block,Binding,FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |