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AF/LE Purified Anti-Human/Mouse IL-5 Antibody[TRFK5]

Uniprot : P05113,P04401

Cat.No.:E-AB-F12050
Applications: ICFCM,Neut
Isotype: Rat IgG1, κ
Host: Rat

Applications:

ICFCM,Neut

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1mg

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Antigen Details

Alternate Names	Interleukin-5,IL-5,B-cell differentiation factor I,T-cell replacing factor,TRF
Swissprot	P05113,P04401
References	1. Fitzgerald, K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Takatsu, K, et al. 1988. Immunol. Rev. 102:107. 3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299. 4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.

Product Details

Form	Liquid
Clone No.	TRFK5(See Other Available Formats)
Host	Rat
Isotype	Rat IgG1, κ
Isotype Control	AF/LE Purified Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F098230]
Reactivity	Human,Mouse
Application	ICFCM,Neut (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Protocols & Troubleshootings

Related Protocols	Staining Intracellular Antigens for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human/Mouse IL-5 Antibody[TRFK5]	25μg, 100μg	Check More
PE Anti-Human/Mouse IL-5 Antibody[TRFK5]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human/Mouse IL-5 Antibody[TRFK5]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human/Mouse IL-5 Antibody[TRFK5]	25μg, 100μg	Check More
APC Anti-Human/Mouse IL-5 Antibody[TRFK5]	25μg, 100μg	Check More

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