AF/LE Purified Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F10160

Uniprot : Q08722,Q61735,P97829
  • Host: Mouse
  • Applications: FCM,WB,IH,IP,ICC&IF
  • Isotype: Mouse IgG1, κ

To Purchase E-AB-F10160

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $75
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Antigen Details

Alternate Names Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP
Swissprot Q08722,Q61735,P97829
References
1. Gresham HD, Dale BM, Potter JW, et al. J Exp Med. 2000; 191(3):515-528.
2. Jiang P, Lagenaur CF, Narayanan V. J Biol Chem. 1999; 274(2):559-562.
3. Lindberg FP, Bullard DC, Caver TE, Gresham HD, Beaudet AL, Brown EJ. Science. 1996; 274(5288):795-798.
4. Lindberg FP, Gresham HD, Schwarz E, Brown EJ. . J Cell Biol. 1993; 123(2):485-496.
5. Mi ZP, Jiang P, Weng WL, Lindberg FP, Narayanan V, Lagenaur CF. J Comp Neurol. 2000; 416(3):335-344.
6. Oldenborg PA, Zheleznyak A, Fang YF, Lagenaur CF, Gresham HD, Lindberg FP. Science. 2000; 288(5473):2051-2054.
7. Reinhold MI, Lindberg FP, Plas D, Reynolds S, Peters MG, Brown EJ. J Cell Sci. 1995 November; 108(Pt 11):3419-3425.

Product Details

Form Liquid
Clone No. MIAP410(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control AF/LE Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F097930]
Reactivity Human, Mouse, Rat
Application FCM,WB,IH,IP,ICC&IF (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F10160 AF/LE Purified Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F10160 50 μg, 500 μg, 1 mg
E-AB-F1016A Purified Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016A 50 μg, 100 μg
E-AB-F1016B Biotin Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016B 50 μg, 100 μg
E-AB-F1016C FITC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016C 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016D PE Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016D 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016E APC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016E 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016F PerCP Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016F 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016G PE/Cyanine5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016G 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016H PE/Cyanine7 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016H 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016I PE/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016I 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016J PerCP/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016J 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016L AF488 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016L 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016M AF647 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016M 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1016UC FITC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UC 25μg, 50μg, 100μg
E-AB-F1016UD PE Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UD 25μg, 50μg, 100μg
E-AB-F1016UE APC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UE 25μg, 50μg, 100μg
E-AB-F1016UF PerCP Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UF 25μg, 50μg, 100μg
E-AB-F1016UG PE/Cyanine5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UG 25μg, 50μg, 100μg
E-AB-F1016UH PE/Cyanine7 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UH 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F1016UI PE/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UI 25μg, 50μg, 100μg
E-AB-F1016UJ PerCP/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UJ 25μg, 50μg, 100μg
E-AB-F1016UL AF488 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UL 25μg, 50μg, 100μg
E-AB-F1016UM AF647 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016UM 25 μg, 50 μg, 100 μg, 200 μg

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