Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F10160
Cat.No.:E-AB-F10160
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Alternate Names | Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP |
Swissprot | Q08722,Q61735,P97829 |
References |
1. Gresham HD, Dale BM, Potter JW, et al. J Exp Med. 2000; 191(3):515-528.
2. Jiang P, Lagenaur CF, Narayanan V. J Biol Chem. 1999; 274(2):559-562. 3. Lindberg FP, Bullard DC, Caver TE, Gresham HD, Beaudet AL, Brown EJ. Science. 1996; 274(5288):795-798. 4. Lindberg FP, Gresham HD, Schwarz E, Brown EJ. . J Cell Biol. 1993; 123(2):485-496. 5. Mi ZP, Jiang P, Weng WL, Lindberg FP, Narayanan V, Lagenaur CF. J Comp Neurol. 2000; 416(3):335-344. 6. Oldenborg PA, Zheleznyak A, Fang YF, Lagenaur CF, Gresham HD, Lindberg FP. Science. 2000; 288(5473):2051-2054. 7. Reinhold MI, Lindberg FP, Plas D, Reynolds S, Peters MG, Brown EJ. J Cell Sci. 1995 November; 108(Pt 11):3419-3425. |
Form | Liquid |
Clone No. | MIAP410(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | AF/LE Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F097930] |
Reactivity | Human,Mouse,Rat |
Application | FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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