Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD115/CSF-1R Antibody[AFS98]
Cat.No.:E-AB-F11070
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Alternate Names | Macrophage colony-stimulating factor 1 receptor,CSF-1 receptor (EC:2.7.10.1),CSF-1-R,CSF-1R,M-CSF-R,Proto-oncogene c-Fms,CD115,Csf1r,Csfmr,Fms |
Swissprot | P09581 |
References |
1. Sudo T, et al. 1995 Oncogene 11:2469.
2. Murayama T, et al. 1999 Circulation 99:1740. 3. Goswami S, et al. 2005 Cancer Res. 65:5278. 4. Yu W, et al. 2008 J. Leuko. Biol. 84:852. |
Form | Liquid |
Clone No. | AFS98(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330] |
Reactivity | Mouse |
Application | FCM,Block,Neut (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
Purified Anti-Mouse CD115/CSF-1R Antibody[AFS98] | 25μg, 100μg | Check More |
PE Anti-Mouse CD115/CSF-1R Antibody[AFS98] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD115/CSF-1R Antibody[AFS98] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse CD115/CSF-1R Antibody[AFS98] | 25μg, 100μg | Check More |
APC Anti-Mouse CD115/CSF-1R Antibody[AFS98] | 25μg, 100μg | Check More |