AF/LE Purified Anti-Mouse CD162 Antibody[4RA10] E-AB-F10340

Uniprot : Q62170
  • Host: Rat
  • Applications: FCM,WB,Block,IHC
  • Isotype: Rat IgG1, κ

To Purchase E-AB-F10340

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $95
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Antigen Details

Alternate Names P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162
Swissprot Q62170
References
1. Borges E, Eytner R, Moll T, et al.Blood. 1997; 90(5):1934-1942.
2. Borges E, Tietz W, Steegmaier M, et al. J Exp Med. 1997; 185(3):573-578.
3. Frenette PS, Denis CV, Weiss L, et al. . J Exp Med. 2000; 191(8):1413-1422.

Product Details

Form Liquid
Clone No. 4RA10(See Other Available Formats)
Host Rat
Isotype Rat IgG1, κ
Isotype Control AF/LE Purified Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F098230]
Reactivity Mouse
Application FCM,WB,Block,IHC (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F10340 AF/LE Purified Anti-Mouse CD162 Antibody[4RA10] E-AB-F10340 50 μg, 500 μg, 1 mg
E-AB-F1034A Purified Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034A 50 μg, 100 μg
E-AB-F1034B Biotin Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034B 50 μg, 100 μg
E-AB-F1034D PE Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034D 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1034E APC Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034E 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1034G PE/Cyanine5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034G 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1034H PE/Cyanine7 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034H 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1034I PE/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034I 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1034J PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034J 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1034L AF488 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034L 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1034M AF647 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034M 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1034UD PE Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UD 25μg, 50μg, 100μg
E-AB-F1034UE APC Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UE 25μg, 50μg, 100μg
E-AB-F1034UG PE/Cyanine5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UG 25μg, 50μg, 100μg
E-AB-F1034UH PE/Cyanine7 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UH 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F1034UI PE/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UI 25μg, 50μg, 100μg
E-AB-F1034UJ PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UJ 25μg, 50μg, 100μg
E-AB-F1034UL AF488 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UL 25μg, 50μg, 100μg
E-AB-F1034UM AF647 Anti-Mouse CD162 Antibody[4RA10] E-AB-F1034UM 25μg, 50μg, 100μg

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