Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD16/32 Antibody[2.4G2]
Cat.No.:E-AB-F09970
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Alternate Names | CD16a/b,CD32,CD32A/B,Fc fragment of IgG low affinity IIa/b receptor,Fc fragment of IgG low affinity IIIa/b receptor,Fc fragment of IgG low affinity IIIb receptor,Fc gamma receptor III A/B,FCG2A,FcGR,FCGR2A/BFCGR3,FCGR3A/B,Fc gamma RIIa/b |
Swissprot | P08508,P08101 |
References |
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355. 3. Qiu WQ, et al. 1990. Science 248:732. |
Form | Liquid |
Clone No. | 2.4G2(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430] |
Reactivity | Mouse |
Application | Block,FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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