Alternate Names	Integrin beta-2,Itgb2,Cell surface adhesion glycoproteins LFA-1/CR3/p150+95 subunit beta,Complement receptor C3 subunit beta,CD18
Swissprot	P11835
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175. 3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Product Details

Form	Liquid
Clone No.	M18/2(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
Biotin Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
FITC Anti-Mouse CD18 Antibody[M18/2]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD18 Antibody[M18/2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD18 Antibody[M18/2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD18 Antibody[M18/2]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
PE Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More

Citations

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