Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD209b Antibody[22D1] E-AB-F10220

AF/LE Purified Anti-Mouse CD209b Antibody[22D1] E-AB-F10220

Uniprot : Q8CJ91

Cat.No.:E-AB-F10220
Applications: FCM
Isotype: Armenian Hamster IgG
Host: Armenian Hamster

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Antigen Details

Alternate Names	CD209 antigen-like protein B,Cd209b,DC-SIGN-related protein 1,DC-SIGNR1,OtB7,CD209,Cd209b
Swissprot	Q8CJ91
References	1. Zhou Y, et al. 2010. Nat. Med. 16:1128. 2. Saunders SP, et al. 2010. J. Immunol. 184:2627. 3. Silva-Martin N, et al. 2009. Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 65:1264. 4. Saunders SP, et al. 2009. Infect. Immun. 77:399. 5. Park JY, et al. 2009. Neurosci. Lett. 450:246. 6. Lanoue A, et al. J. Exp. Med. 200:1383.

Product Details

Form	Liquid
Clone No.	22D1(See Other Available Formats)
Host	Armenian Hamster
Isotype	Armenian Hamster IgG
Isotype Control	AF/LE Purified Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F098530]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD209B, also known as SIGN-R1, is a mouse C-type lectin receptor predominantly expressed on macrophages in the spleen marginal zone and lymph nodes medulla. CD209B is a mouse homolog of human CD209/DC-SIGN and is involved in innate immune response. CD209B mediates the recognition and uptake of pathogen products, such as lipopolysaccharides (LPS), pneumococcal polysaccharides, and dextrans. CD209B has been demonstrated to facilitate the clearance of encapsulated pneumococcus by directly binding to C1q and activating complement through an immunoglobulin independent pathway.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD209b Antibody[22D1] E-AB-F1022A	25μg, 100μg	Check More
Biotin Anti-Mouse CD209b Antibody[22D1] E-AB-F1022B	25μg, 100μg	Check More

Citations

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