Purified Anti-Mouse CD22 Antibody[Cy34.1], Functional Grade (E-AB-F10210)
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For research use only.
| Alternate Names | Lyb, SIGLEC2FLJ, B-cell receptor CD, T-cell surface antigen Leu, sialic acid binding Ig-like lectin, Sialic acid-binding Ig-like lectin, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, CD22 antigenMGC130020, CD22 molecule, sialic acid binding Ig-like lectin 2, Sialic acid-binding Ig-like lectin 2, Siglec-2, SIGLEC2FLJ22814, T-cell surface antigen Leu-14, Lyb-8, Siglec2, Cd22, Lyb8, Siglec-2 |
| Clone | |
| Leadtime | Inquire |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Depletion Reported in the literature |
| Background | CD22 is a member of the sialoadhesion family.CD22 is expressed on the surface of a subpopulation of mature B lymphocytes and may modulate signaling through the B cell antigen receptor. The OX-97 monoclonal antibody recognizes the second domain of the CD22. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse CD22 protein |
| Abbre | CD22 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG1, κ |
| Isotype Control | Purified Mouse IgG1, κ Isotype Control[MOPC-21], Functional Grade |
| Applications |
FCM;Depletion
|
| Research Areas | Immunology |
| Cellular Localization |
Cell membrane
|
| Tissue Specificity |
B-lymphocytes.
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Petter Säwén, et al. Cell Rep. 2016 Mar 29;14(12):2809-18. |
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