|Alternate Names||B- and T-lymphocyte attenuator,Btla,B- and T-lymphocyte-associated protein,CD272,Btla|
1. Liu X, et al. 2009. J. Immunol. 182:4516.
2. Miller ML, et al. 2009. J. Immunol. 183:32.
3. Sun Y, et al. 2009. J. Immunol. 183:1946.
4. Vendel AC, et al. 2009. J. Immunol. 182:1509.
5. Watanabe N, et al. 2003. Nat. Immunol. 4:670.
|Clone No.||PK18.6(See Other Available Formats)|
|Isotype||Rat IgG1, κ|
|Isotype Control||AF/LE Purified Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F098230]|
|Application||FCM (See the Application Description)|
|Storage Buffer||0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.|
|Recommended Use||Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 μg per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.|
|Shipping||Biological ice pack at 4℃|
|Stability & Storage||Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
|Conjugation||None (Purified antibody/Azide Free/Low endotoxin)|
|CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm. The existence of three distinct BTLA alleles has been reported.|
|Related Protocols||Staining Cell Surface Targets for Flow Cytometry|
|E-AB-F10240||AF/LE Purified Anti-Mouse CD272 Antibody[PK18.6] E-AB-F10240||50 μg, 500 μg, 1 mg|
|E-AB-F1024A||Purified Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024A||50 μg, 100 μg|
|E-AB-F1024B||Biotin Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024B||50 μg, 100 μg|
|E-AB-F1024E||APC Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024E||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1024UE||APC Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024UE||25μg, 50μg, 100μg|
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