AF/LE Purified Anti-Mouse CD272 Antibody[PK18.6] E-AB-F10240

Uniprot : Q7TSA3
  • Host: Rat
  • Applications: FCM
  • Isotype: Rat IgG1, κ

To Purchase E-AB-F10240

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $95
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Antigen Details

Alternate Names B- and T-lymphocyte attenuator,Btla,B- and T-lymphocyte-associated protein,CD272,Btla
Swissprot Q7TSA3
References
1. Liu X, et al. 2009. J. Immunol. 182:4516.
2. Miller ML, et al. 2009. J. Immunol. 183:32.
3. Sun Y, et al. 2009. J. Immunol. 183:1946.
4. Vendel AC, et al. 2009. J. Immunol. 182:1509.
5. Watanabe N, et al. 2003. Nat. Immunol. 4:670.

Product Details

Form Liquid
Clone No. PK18.6(See Other Available Formats)
Host Rat
Isotype Rat IgG1, κ
Isotype Control AF/LE Purified Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F098230]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm. The existence of three distinct BTLA alleles has been reported.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F10240 AF/LE Purified Anti-Mouse CD272 Antibody[PK18.6] E-AB-F10240 50 μg, 500 μg, 1 mg
E-AB-F1024A Purified Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024A 50 μg, 100 μg
E-AB-F1024B Biotin Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024B 50 μg, 100 μg
E-AB-F1024D PE Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024D 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1024E APC Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024E 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1024UD PE Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024UD 25μg, 50μg, 100μg
E-AB-F1024UE APC Anti-Mouse CD272 Antibody[PK18.6] E-AB-F1024UE 25μg, 50μg, 100μg

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