Current Position:Home>>Antibodies>>FCM Antibodies>> AF/LE Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F11320
|Alternate Names||B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1|
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.
|Clone No.||10F.9G2(See Other Available Formats)|
|Isotype||Rat IgG2b, κ|
|Isotype Control||AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430]|
|Application||FCM,IHC,IF,Block (See the Application Description)|
|Storage Buffer||0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.|
|Recommended Use||Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.|
|Shipping||Biological ice pack at 4℃|
|Stability & Storage||Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
|Conjugation||None (Purified antibody/Azide Free/Low endotoxin)|
|CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.|
|Related Protocols||Staining Cell Surface Targets for Flow Cytometry|
|E-AB-F11320||AF/LE Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F11320||50 μg, 500 μg, 1 mg|
|E-AB-F1132A||Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132A||50 μg, 100 μg|
|E-AB-F1132B||Biotin Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132B||50 μg, 100 μg|
|E-AB-F1132C||FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132C||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1132D||PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132D||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1132E||APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132E||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1132I||PE/Cyanine5.5 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132I||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1132UC||FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132UC||25 μg, 50 μg, 100 μg, 200 μg|
|E-AB-F1132UD||PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132UD||25μg, 50μg, 100μg|
|E-AB-F1132UE||APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132UE||25μg, 50μg, 100μg|
|E-AB-F1132UI||PE/Cyanine5.5 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132UI||25μg, 50μg, 100μg|