AF/LE Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F11320

Uniprot : Q9EP73
  • Host: Rat
  • Applications: FCM,IHC,IF,Block
  • Isotype: Rat IgG2b, κ

To Purchase E-AB-F11320

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $75
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Antigen Details

Alternate Names B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1
Swissprot Q9EP73
References
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Product Details

Form Liquid
Clone No. 10F.9G2(See Other Available Formats)
Host Rat
Isotype Rat IgG2b, κ
Isotype Control AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430]
Reactivity Mouse
Application FCM,IHC,IF,Block (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F11320 AF/LE Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F11320 50 μg, 500 μg, 1 mg
E-AB-F1132A Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132A 50 μg, 100 μg
E-AB-F1132B Biotin Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132B 50 μg, 100 μg
E-AB-F1132C FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132C 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1132D PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132D 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1132E APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132E 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1132I PE/Cyanine5.5 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132I 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1132UC FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132UC 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F1132UD PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132UD 25μg, 50μg, 100μg
E-AB-F1132UE APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132UE 25μg, 50μg, 100μg
E-AB-F1132UI PE/Cyanine5.5 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] E-AB-F1132UI 25μg, 50μg, 100μg

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