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AF/LE Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12]

Uniprot : Q02242

Cat.No.:E-AB-F11310
Applications: Block,Neut,FCM
Isotype: Rat IgG2a, κ
Host: Rat

Applications:

Block,Neut,FCM

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500μg
1mg

Price: $95

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Antigen Details

Alternate Names	PD-1, Programmed Death-1
Swissprot	Q02242
References	1. Nishimura H, et al. 2001. Science 291:319 2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2008. J Immunol. 181:5313

Product Details

Form	Liquid
Clone No.	29F.1A12(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity	Mouse
Application	Block,Neut,FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
Biotin Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More

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