Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD28 Antibody[37.51]
Cat.No.:E-AB-F10260
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Alternate Names | T-cell-specific surface glycoprotein CD28,Cd28,CD28 |
Swissprot | P31041 |
References |
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380. |
Form | Liquid |
Clone No. | 37.51(See Other Available Formats) |
Host | Syrian Hamster |
Isotype | Syrian Hamster IgG |
Isotype Control | AF/LE Purified Syrian Hamster IgG Isotype Control[SHG-1] [Product E-AB-F097630] |
Reactivity | Mouse |
Application | Activ,Block,FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
Purified Anti-Mouse CD28 Antibody[37.51] | 25μg, 100μg | Check More |
PE Anti-Mouse CD28 Antibody[37.51] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD28 Antibody[37.51] | 50Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Mouse CD28 Antibody[37.51] | 50Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 647 Anti-Mouse CD28 Antibody[37.51] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse CD28 Antibody[37.51] | 25μg, 100μg | Check More |
APC Anti-Mouse CD28 Antibody[37.51] | 25μg, 100μg | Check More |
Elab Fluor® 488 Anti-Mouse CD28 Antibody[37.51] | 25μg, 100μg | Check More |
Elab Fluor® 647 Anti-Mouse CD28 Antibody[37.51] | 25μg, 100μg | Check More |
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