AF/LE Purified Anti-Mouse CD3 Antibody[17A2] E-AB-F10130

  • Host: Rat
  • Applications: FCM,IHC-F,IP,ICC
  • Isotype: Rat IgG2b, κ

To Purchase E-AB-F10130

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $75
Qty:

Antigen Details

Alternate Names T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain,CD3E/D/G/Z,CD3e/d/g/z,CD3E/D/G/Z,CD3
Swissprot P04235,P11942,P22646,P24161,P29020
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Product Details

Form Liquid
Clone No. 17A2(See Other Available Formats)
Host Rat
Isotype Rat IgG2b, κ
Isotype Control AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430]
Reactivity Mouse
Application FCM,IHC-F,IP,ICC (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F10130 AF/LE Purified Anti-Mouse CD3 Antibody[17A2] E-AB-F10130 50 μg, 500 μg, 1 mg
E-AB-F1013A Purified Anti-Mouse CD3 Antibody[17A2] E-AB-F1013A 50 μg, 100 μg
E-AB-F1013B Biotin Anti-Mouse CD3 Antibody[17A2] E-AB-F1013B 50 μg, 100 μg
E-AB-F1013C FITC Anti-Mouse CD3 Antibody[17A2] E-AB-F1013C 100 Tests, 200 Tests, 500 Tests
E-AB-F1013D PE Anti-Mouse CD3 Antibody[17A2] E-AB-F1013D 100 Tests, 200 Tests, 500 Tests
E-AB-F1013E APC Anti-Mouse CD3 Antibody[17A2] E-AB-F1013E 100 Tests, 200 Tests, 500 Tests
E-AB-F1013F PerCP Anti-Mouse CD3 Antibody[17A2] E-AB-F1013F 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1013G PE/Cyanine5 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013G 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1013H PE/Cyanine7 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013H 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1013I PE/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013I 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1013J PerCP/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013J 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1013L AF488 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013L 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1013M AF647 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013M 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1013UC FITC Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UC 50μg, 100μg
E-AB-F1013UD PE Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UD 25μg, 50μg, 100μg
E-AB-F1013UE APC Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UE 25μg, 50μg, 100μg
E-AB-F1013UF PerCP Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UF 25μg, 50μg, 100μg
E-AB-F1013UG PE/Cyanine5 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UG 25μg, 50μg, 100μg
E-AB-F1013UH PE/Cyanine7 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UH 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F1013UI PE/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UI 25μg, 50μg, 100μg
E-AB-F1013UJ PerCP/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UJ 25μg, 50μg, 100μg
E-AB-F1013UL AF488 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UL 25μg, 50μg, 100μg
E-AB-F1013UM AF647 Anti-Mouse CD3 Antibody[17A2] E-AB-F1013UM 25μg, 50μg, 100μg

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