Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F11030
Cat.No.:E-AB-F11030
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Alternate Names | T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E |
Swissprot | P22646 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263. |
Form | Liquid |
Clone No. | 145-2C11(See Other Available Formats) |
Host | Armenian Hamster |
Isotype | Armenian Hamster IgG |
Isotype Control | AF/LE Purified Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F098530] |
Reactivity | Mouse |
Application | Activ,Depletion,FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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