AF/LE Purified Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F10280

Uniprot : P27512
  • Host: Rat
  • Applications: FCM
  • Isotype: Rat IgG2a, κ

To Purchase E-AB-F10280

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $75
Qty:

Antigen Details

Alternate Names Tumor necrosis factor receptor superfamily member 5,Cd40,B-cell surface antigen CD40,Bp50,CD40L receptor,CD40,Tnfrsf5
Swissprot P27512
References
1. Hasbold J, Johnson-Leger C, Atkins CJ, Clark EA, Klaus GG. Eur J Immunol. 1994; 24(8):1835-1842.
2. Hasbold J, Klaus GG. Eur J Immunol. 1994; 24(1):152-157.
3. Klaus GG, Holman M, Hasbold J. Eur J Immunol. 1994; 24(11):2714-2719.
4. Noelle RJ, Ledbetter JA, Aruffo A. Immunol Today. 1992; 13(11):431-433.
5. Parry SL, Hasbold J, Holman M, Klaus GG. J Immunol. 1994; 152(6):2821-2829.

Product Details

Form Liquid
Clone No. FGK4.5/FGK45(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F10280 AF/LE Purified Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F10280 50 μg, 500 μg, 1 mg
E-AB-F1028A Purified Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028A 50 μg, 100 μg
E-AB-F1028B Biotin Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028B 50 μg, 100 μg
E-AB-F1028C FITC Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028C 20 Tests, 50 Tests, 100 Tests
E-AB-F1028D PE Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028D 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1028E APC Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028E 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1028G PE/Cyanine5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028G 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1028H PE/Cyanine7 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028H 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1028I PE/Cyanine5.5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028I 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1028J PerCP/Cyanine5.5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028J 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1028L AF488 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028L 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1028M AF647 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028M 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1028UC FITC Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UC 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F1028UD PE Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UD 25μg, 50μg, 100μg
E-AB-F1028UE APC Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UE 25μg, 50μg, 100μg
E-AB-F1028UG PE/Cyanine5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UG 25μg, 50μg, 100μg
E-AB-F1028UH PE/Cyanine7 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UH 25μg, 50μg, 100μg
E-AB-F1028UI PE/Cyanine5.5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UI 25μg, 50μg, 100μg
E-AB-F1028UJ PerCP/Cyanine5.5 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UJ 25μg, 50μg, 100μg
E-AB-F1028UL AF488 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UL 25μg, 50μg, 100μg
E-AB-F1028UM AF647 Anti-Mouse CD40 Antibody[FGK4.5/FGK45] E-AB-F1028UM 25μg, 50μg, 100μg

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