Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD45.2 Antibody[104.2] E-AB-F11220

AF/LE Purified Anti-Mouse CD45.2 Antibody[104.2] E-AB-F11220

Cat.No.:E-AB-F11220
Applications: Block,FCM
Isotype: Mouse IgG2a, κ
Host: Mouse

Applications:

Block,FCM

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Antigen Details

Alternate Names	Ly-5.2, LCA
References	1. Suzuki K, et al. 2000. Immunity 13:691.

Product Details

Form	Liquid
Clone No.	104.2(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a, κ
Isotype Control	AF/LE Purified Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F098030]
Reactivity	Mouse
Application	Block,FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122A	25μg, 100μg	Check More
Biotin Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122B	25μg, 100μg	Check More
FITC Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122C	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122E	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122G	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122H	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122I	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122J	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD45.2 Antibody[104.2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD45.2 Antibody[104.2]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122UC	25μg, 100μg	Check More
PE Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122UD	25μg, 100μg	Check More
APC Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122UE	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122UG	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122UH	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122UI	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD45.2 Antibody[104.2] E-AB-F1122UJ	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD45.2 Antibody[104.2]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD45.2 Antibody[104.2]	25μg, 100μg	Check More

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