Purified Anti-Mouse CD4 Antibody[GK1.5], Functional Grade (E-AB-F10970)
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For research use only.
| Alternate Names | L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, T4 |
| Clone | |
| Leadtime | Order now, ship in 3 days |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Depletion Reported in the literature |
| Background | CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse CD4 protein |
| Abbre | CD4 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG2b, κ |
| Isotype Control | Purified Rat IgG2b, κ Isotype Control[LTF-2], Functional Grade |
| Applications |
FCM;Depletion
|
| Research Areas | Immunology |
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Kelly D Moynihan, et al. Nat Med. 2016 Dec;22(12):1402-1410. |
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