AF/LE Purified Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F10180

Uniprot : P13597
  • Host: Rat
  • Applications: FCM,IF
  • Isotype: Rat IgG2b, κ

To Purchase E-AB-F10180

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $75
Qty:

Antigen Details

Alternate Names Intercellular adhesion molecule 1,Icam1,MALA-2,MyD10,CD54,Icam-1
Swissprot P13597
References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.

Product Details

Form Liquid
Clone No. YN1/1.7.4(See Other Available Formats)
Host Rat
Isotype Rat IgG2b, κ
Isotype Control AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430]
Reactivity Mouse
Application FCM,IF (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F10180 AF/LE Purified Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F10180 50 μg, 500 μg, 1 mg
E-AB-F1018A Purified Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018A 50 μg, 100 μg
E-AB-F1018B Biotin Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018B 50 μg, 100 μg
E-AB-F1018C FITC Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018C 100 Tests, 200 Tests, 500 Tests
E-AB-F1018D PE Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018D 100 Tests, 200 Tests, 500 Tests
E-AB-F1018E APC Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018E 100 Tests, 200 Tests, 500 Tests
E-AB-F1018F PerCP Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018F 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1018G PE/Cyanine5 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018G 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1018H PE/Cyanine7 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018H 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1018I PE/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018I 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1018J PerCP/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018J 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1018L AF488 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018L 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1018M AF647 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018M 50 Tests, 100 Tests, 200 Tests, 500 Tests
E-AB-F1018UC FITC Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UC 25μg, 50μg, 100μg
E-AB-F1018UD PE Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UD 25μg, 50μg, 100μg
E-AB-F1018UE APC Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UE 25μg, 50μg, 100μg
E-AB-F1018UF PerCP Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UF 25μg, 50μg, 100μg
E-AB-F1018UG PE/Cyanine5 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UG 25μg, 50μg, 100μg
E-AB-F1018UH PE/Cyanine7 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UH 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F1018UI PE/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UI 25μg, 50μg, 100μg
E-AB-F1018UJ PerCP/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UJ 25μg, 50μg, 100μg
E-AB-F1018UL AF488 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UL 25μg, 50μg, 100μg
E-AB-F1018UM AF647 Anti-Mouse CD54 Antibody[YN1/1.7.4] E-AB-F1018UM 25μg, 50μg, 100μg

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
How do you know
Elabscience:

*Verification code: