Alternate Names	L-selectin,Sell,CD62 antigen-like family member L,LAM-1,LECAM1,Lymph node homing receptor,Ly-22, CD62L,Lnhr,Ly22
Swissprot	P18337
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. 3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Product Details

Form	Liquid
Clone No.	Mel14(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity	Mouse
Application	Neut,FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte 'rolling' on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
Biotin Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
FITC Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD62L Antibody[Mel14]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
PE Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
APC Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD62L Antibody[Mel14]	25μg, 100μg	Check More

Citations

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