Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD80 Antibody[16-10A1] E-AB-F09920

AF/LE Purified Anti-Mouse CD80 Antibody[16-10A1] E-AB-F09920

Uniprot : Q00609

Cat.No.:E-AB-F09920
Applications: Block,FCM
Isotype: Armenian Hamster IgG
Host: Armenian Hamster

Applications:

Block,FCM

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1mg

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Antigen Details

Alternate Names	T-lymphocyte activation antigen CD80,Cd80,Activation B7-1 antigen,B7
Swissprot	Q00609
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Product Details

Form	Liquid
Clone No.	16-10A1(See Other Available Formats)
Host	Armenian Hamster
Isotype	Armenian Hamster IgG
Isotype Control	AF/LE Purified Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F098530]
Reactivity	Mouse
Application	Block,FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992A	25μg, 100μg	Check More
Biotin Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992B	25μg, 100μg	Check More
FITC Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992C	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992E	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992G	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992H	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992I	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992J	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD80 Antibody[16-10A1]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UC	25μg, 100μg	Check More
PE Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UD	25μg, 100μg	Check More
APC Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UE	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UG	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UH	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UI	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UJ	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD80 Antibody[16-10A1]	25μg, 100μg	Check More

Citations

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