Alternate Names	T-lymphocyte activation antigen CD86,Cd86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1
Swissprot	P42082
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Hathcock KS, et al. 1993. Science 262:905. 3. Freeman GJ, et al. 1993. Science 262:907. 4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Product Details

Form	Liquid
Clone No.	GL-1(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Biotin Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
FITC Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
APC Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More

Citations

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