AF/LE Purified Anti-Mouse CD86 Antibody[GL-1] E-AB-F09940

Uniprot : P42082
  • Host: Rat
  • Applications: FCM,IHC-F,IP,ICC
  • Isotype: Rat IgG2a, κ

To Purchase E-AB-F09940

Size:
  • 50 μg
  • 500 μg
  • 1 mg
Price: $75
Qty:

Antigen Details

Alternate Names T-lymphocyte activation antigen CD86,Cd86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1
Swissprot P42082
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Product Details

Form Liquid
Clone No. GL-1(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity Mouse
Application FCM,IHC-F,IP,ICC (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 g per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F09940 AF/LE Purified Anti-Mouse CD86 Antibody[GL-1] E-AB-F09940 50 μg, 500 μg, 1 mg
E-AB-F0994A Purified Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994A 50 μg, 100 μg
E-AB-F0994B Biotin Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994B 50 μg, 100 μg
E-AB-F0994C FITC Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994C 50 Tests, 100 Tests, 500 Tests
E-AB-F0994D PE Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994D 50 Tests, 100 Tests, 500 Tests
E-AB-F0994E APC Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994E 50 Tests, 100 Tests, 500 Tests
E-AB-F0994G PE/Cyanine5 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994G 20 Tests, 50 Tests, 100 Tests, 500 Tests
E-AB-F0994H PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994H 20 Tests, 50 Tests, 100 Tests, 500 Tests
E-AB-F0994I PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994I 20 Tests, 50 Tests, 100 Tests, 500 Tests
E-AB-F0994J PerCP/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994J 20 Tests, 50 Tests, 100 Tests, 500 Tests
E-AB-F0994L AF488 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994L 20 Tests, 50 Tests, 100 Tests, 500 Tests
E-AB-F0994M AF647 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994M 20 Tests, 50 Tests, 100 Tests, 500 Tests
E-AB-F0994UC FITC Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UC 25μg, 50μg, 100μg
E-AB-F0994UD PE Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UD 25μg, 50μg, 100μg
E-AB-F0994UE APC Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UE 25μg, 50μg, 100μg
E-AB-F0994UG PE/Cyanine5 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UG 25μg, 50μg, 100μg
E-AB-F0994UH PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UH 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F0994UI PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UI 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F0994UJ PerCP/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UJ 25μg, 50μg, 100μg
E-AB-F0994UL AF488 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UL 25 μg, 50 μg, 100 μg, 200 μg
E-AB-F0994UM AF647 Anti-Mouse CD86 Antibody[GL-1] E-AB-F0994UM 25μg, 50μg, 100μg

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