Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD8a Antibody[53-6.7] E-AB-F11040

AF/LE Purified Anti-Mouse CD8a Antibody[53-6.7] E-AB-F11040

Uniprot : P01731

Cat.No.:E-AB-F11040
Applications: Block,Depletion,FCM
Isotype: Rat IgG2a, κ
Host: Rat

Applications:

Block,Depletion,FCM

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Antigen Details

Alternate Names	T-cell surface glycoprotein CD8 alpha chain,CD8A,T-lymphocyte differentiation antigen T8/Leu-2,MAL
Swissprot	P01731
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Zamoyska R. 1994. Immunity 1:243. 3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Product Details

Form	Liquid
Clone No.	53-6.7(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity	Mouse
Application	Block,Depletion,FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104A	25μg, 100μg	Check More
Biotin Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104B	25μg, 100μg	Check More
FITC Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104C	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104E	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104F	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104H	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104I	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104J	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104UC	25μg, 100μg	Check More
PE Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104UD	25μg, 100μg	Check More
APC Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104UE	25μg, 100μg	Check More
PerCP Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104UF	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104UH	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104UI	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7] E-AB-F1104UJ	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More

Citations

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