Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]
Cat.No.:E-AB-F10940
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Alternate Names | Thy-1.2 membrane glycoprotein,Thy1.2,Thy-1.2 antigen,CD90.2,Thy-1.2 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331. 3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1. 4. Mayani H, et al. 1994. Blood 83:2410. |
Form | Liquid |
Clone No. | 30H12(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430] |
Reactivity | Mouse |
Application | Depletion,FCM,Apoptosis (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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