Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]

AF/LE Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]

Cat.No.:E-AB-F10940
Applications: Depletion,FCM,Apoptosis
Isotype: Rat IgG2b, κ
Host: Rat

Applications:

Depletion,FCM,Apoptosis

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Antigen Details

Alternate Names	Thy-1.2 membrane glycoprotein,Thy1.2,Thy-1.2 antigen,CD90.2,Thy-1.2
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Craig W, et al. 1993. J. Exp. Med. 177:1331. 3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1. 4. Mayani H, et al. 1994. Blood 83:2410.

Product Details

Form	Liquid
Clone No.	30H12(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430]
Reactivity	Mouse
Application	Depletion,FCM,Apoptosis (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
Biotin Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
FITC Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
PE Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
APC Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
PerCP Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]	25μg, 100μg	Check More

Citations

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