Alternate Names	Adhesion G protein-coupled receptor E1,Adgre1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,Adgre1,Emr1, Gpf480
Swissprot	Q61549
References	1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Product Details

Form	Liquid
Clone No.	CI:A3-1(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430]
Reactivity	Mouse
Application	FCM,FA (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Biotin Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
FITC Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
APC Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More

Citations

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