Purified Anti-Mouse IL-6 Antibody[MP5-20F3], Functional Grade (E-AB-F12070)
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For research use only.
| Alternate Names | BSF, Interleukin, Interleukin HP, IFNB, B-cell hybridoma growth factor, BSF2, CDF, HGF, HSF, IFNB2, IL 6, Interleukin HP-1, Interleukin-6, Il-6, Il6 |
| Clone | |
| Leadtime | Order now, ship in 3 days |
| Verified Samples | Verified Samples in FCM: HEK293T cells transfected with pcDNA3.1 plasmid encoding Mouse IL-6 gene |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Neut Reported in the literature |
| Background | Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Human IL-6 protein |
| Abbre | IL-6 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Isotype | Rat IgG1, κ |
| Isotype Control | Purified Rat IgG1, κ Isotype Control[HRPN], Functional Grade |
| Applications |
FCM;Neut
|
| Research Areas | Immunology;Innate Immunity |
| Cellular Localization |
Secreted
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Hirotake Tsukamoto, et al. Nat Commun. 2015 Apr 7:6:6702. |
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