AKAP17A Polyclonal Antibody (E-AB-19042)

For research use only.
Verified Samples |
Verified Samples in WB: 293T |
Dilution | WB 1:500-1:2000 |
Isotype | IgG |
Host | Rabbit |
Reactivity | Human |
Applications | WB |
Clonality | Polyclonal |
Immunogen | Fusion protein of human AKAP17A |
Abbre | AKAP17A |
Synonyms | 721P, A-kinase anchor protein 17A, AK17A, AKAP-17A, AKAP17A, B lymphocyte antigen, B lymphocyte surface antigen, B-lymphocyte antigen, CCDC133, PRKA17A, Protein XE7, Protein kinase A-anchoring protein 17A, Pseudoautosomal gene XE7, SFRS 17A, Sp, arginine/serine-rich 17A |
Swissprot | |
Calculated MW | 81 kDa |
Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus speckle. |
Concentration | 0.84 mg/mL |
Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
Purification Method | Antigen affinity purification |
Research Areas | Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | This locus encodes a protein kinase A anchoring protein. The encoded protein is part of the spliceosome complex and is involved in the regulation of alternate splicing in some mRNA precursors. Alternatively spliced transcript variants have been identified for this gene.AKAP17A (A-Kinase Anchoring Protein 17A) is a Protein Coding gene. Diseases associated with AKAP17A include Null-Cell Leukemia and Chronic Tic Disorder. GO annotations related to this gene include poly(A) RNA binding and protein kinase A binding. |
Other Clones
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Unconjugated
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